Sajid Hussain *, Farhat Ullah, Abdul Sadiq , Muhammad Ayaz , Azhar-ul-Haq Ali Shah, Syed Adnan Ali Shah , Syed Majid Shah , Akhtar Nadhman, Farman Ullah , Abdul Wadood and Mohamed El-Shazly Pages 2805 - 2813 ( 9 )
Background: Liver cancer is a devastating cancer with increasing incidence and mortality rates worldwide. Plants possess numerous therapeutic properties, therefore the search for novel, naturally occurring cytotoxic compounds is urgently needed.Methods: The anticancer activity of plant extracts and isolated compounds from Anchusa arvensis (A. arvensis) were studied against the cell culture of HepG-2 (human hepatocellular carcinoma cell lines) using 3-(4,5-Dimethylthiazol-yl)-diphenyl tetrazoliumbromide (MTT) assay. Apoptosis was investigated by performing Acridine orange –ethidium bromide staining, styox green assay and DNA interaction study. We also used tools for computational chemistry studies of isolated compounds with the tyrosine kinase. Results: In MTT assay, the crude extract caused a significant cytotoxic effect with IC50 of 34.14 ± 0.9 μg/ml against HepG-2 cell lines. Upon fractionation, chloroform fraction (Aa.Chm) exhibited the highest antiproliferative activity with IC50 6.55 ± 1.2 μg/ml followed by ethyl acetate (Aa.Et) fraction (IC50, 24.59 ± 0.85 μg/ml) and n-hexane (Aa.Hex) fraction (IC50 29.53 ± 1.5μg/ml). However, the aqueous (Aa.Aq) fraction did not show any anti-proliferative activity. Bioactivity-guided isolation led to the isolation of two compounds which were characterized as para–methoxycatechol (1) and decane (2) through various spectroscopic techniques. Against HepG-2 cells, compound 1 showed marked potency with IC50 6.03 ± 0.75 μg/ml followed by 2 with IC50 18.52 ± 1.9 μg/ml. DMSO was used as a negative control and doxorubicin as a reference standard (IC50 1.3 ± 0.21 μg/ml). It was observed that compounds 1-2 caused apoptotic cell death evaluated by Acridine orange –ethidium bromide staining, styox green assay and DNA interaction study, therefore both compounds were tested for molecular docking studies against tyrosine kinase to support cytotoxic activity. Conclusion: This study revealed that the plant extracts and isolated compounds possess promising antiproliferative activity against HepG-2 cell lines via apoptotic cell death.
Anchusa arvensis, Cytotoxicity, 3-(4, 5-Dimethylthiazol-yl)-diphenyl tetrazoliumbromide, Anticancer activity, HepG-2 cell lines, Para–methoxycatechol, Decane.
Department of Pharmacy, University of Malakand, Malakand, Department of Pharmacy, University of Malakand, Malakand, Department of Pharmacy, University of Malakand, Malakand, Department of Pharmacy, University of Malakand, Malakand, Department of Chemistry, Kohat University of Science & Technology, Kohat, Research Institute of Natural Products for Drug Discovery (RiND), Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Shah Alam, Department of Pharmacy, University of Malakand, Malakand, Institute of Integrative Biosciences IIB, CECOS University, Peshawar, Department of Pharmacy, Kohat University of Science & Technology, Kohat, Department of Biochemistry, Abdul Wali Khan University Mardan, Mardan, Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, Ain-Shams University, Cairo