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LC/MS/MS Profiling of Tissue Oxysterols and its Application in Dextran Sodium Sulphate Induced Mouse Colitis Models

[ Vol. 17 , Issue. 24 ]

Author(s):

Jiejun Wu*, J.Eric McDuffie*, Jiao Song, Michael C. Harris, Holly Raymond, Yafei Chen, Leslie Nguyen, Steven Nguyen, Justin Kanerva, Heather M. McAllister, Xiaohu Deng, Neelakandha Mani, Changlu Liu and Siquan Sun   Pages 2781 - 2790 ( 10 )

Abstract:


We have developed a workflow to extract, separate, and semi-quantify bioactive oxysterols from mouse colon tissues and fecal matters using solid- and liquid-phase extractions, enzymatic and chemical modifications, and stable-isotope dilution LC/MS/MS. The method was applied to a dextran sodium sulphate (DSS)-induced mouse colitis model, which revealed that one particular dihydroxycholesterol (diOHC), 7α,25-diOHC, was significantly elevated in both colon tissue and fecal matters of mice with colitis compared to that in naïve mice. The extent of 7α,25-diOHC elevation was positively correlated with colitis severity.

Keywords:

Oxysterol separation, Oxysterol quantification, Oxysterol analysis, Dihydroxycholesterols, Mouse colitis biomarker.

Affiliation:

Janssen R&D, LLC., 3210 Merryfield Row, San Diego, CA 92121, Janssen R&D, LLC., 3210 Merryfield Row, San Diego, CA 92121, Janssen Research and Development, LLC, 3210 Merryfield Row, San Diego, CA 92121, Janssen Research and Development, LLC, 3210 Merryfield Row, San Diego, CA 92121, Janssen Research and Development, LLC, 3210 Merryfield Row, San Diego, CA 92121, Janssen Research and Development, LLC, 3210 Merryfield Row, San Diego, CA 92121, Janssen Research and Development, LLC, 3210 Merryfield Row, San Diego, CA 92121, Janssen Research and Development, LLC, 3210 Merryfield Row, San Diego, CA 92121, Janssen Research and Development, LLC, 3210 Merryfield Row, San Diego, CA 92121, Janssen Research and Development, LLC, 3210 Merryfield Row, San Diego, CA 92121, Janssen Research and Development, LLC, 3210 Merryfield Row, San Diego, CA 92121, Janssen Research and Development, LLC, 3210 Merryfield Row, San Diego, CA 92121, Janssen Research and Development, LLC, 3210 Merryfield Row, San Diego, CA 92121, Janssen Research and Development, LLC, 3210 Merryfield Row, San Diego, CA 92121

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