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Antimicrobial Activity of Different Antimicrobial Peptides (AMPs) Against Clinical Methicillin-resistant Staphylococcus aureus (MRSA)

[ Vol. 18 , Issue. 24 ]

Author(s):

Eleonora Ciandrini, Gianluca Morroni, Daniela Arzeni, Wojciech Kamysz, Damian Neubauer, Elzbieta Kamysz, Oscar Cirioni, Lucia Brescini, Wally Baffone and Raffaella Campana*   Pages 2116 - 2126 ( 11 )

Abstract:


Background: Antimicrobial research is being focused to look for more effective therapeutics against antibiotic-resistant infections caused by methicillin-resistant Staphylococcus aureus (MRSA). In this direction, antimicrobial peptides (AMP) appear as promising tool.

Objectives: This study evaluated the antimicrobial activity of different AMPs (Citropin 1.1, Temporin A, Pexiganan, CA(1–7)M(2–9)NH2, Pal-KGK-NH2, Pal-KKKK-NH2, LL-37) against human MRSA clinical isolates.

Methods: The Minimum Inhibitory Concentration (MIC) was assessed for each AMP; then, the most active ones (Citropin 1.1, Temporin A, CA(1–7)M(2–9)NH2 and Pal-KGK-NH2) were tested against selected MRSA strains by time-kill studies.

Results: The lowest MIC value was observed for Pal-KGK-NH2 (1 µg/ml), followed by Temporin A (4- 16 µg/ml), CA(1–7)M(2–9)NH2 (8-16 µg/ml) and Citropin 1.1 (16-64 µg/ml), while higher MICs were evidenced for LL-37, Pexiganan and Pal-KKKK-NH2 (> 128 µg/ml). In time-kill experiments, Citropin 1.1 and CA(1-7)M(2-9)NH2 showed a relatively high percentage of growth inhibition (>30 %) for all the tested MRSA clinical isolates, with a dose-dependent activity resulting in the highest percentage of bacterial growth inhibition (89.39%) at 2MIC concentration.

Conclusion: Overall, our data demonstrated the potential of some AMPs against MRSA isolates, such as Citropin 1.1 and CA(1-7)M(2-9)NH2, that represents a promising area of development for different clinical applications.

Keywords:

AMPs, Methicillin-resistant, Staphylococcus aureus, Clinical isolates, Antimicrobial activity, Time-kill.

Affiliation:

Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Infectious Disease Clinic, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, Infectious Disease Clinic, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, Faculty of Pharmacy, Department of Inorganic Chemistry, Medical University of Gdansk, Gdansk, Faculty of Pharmacy, Department of Inorganic Chemistry, Medical University of Gdansk, Gdansk, Faculty of Chemistry, Department of Molecular Biotechnology, University of Gdansk, Gdansk, Infectious Disease Clinic, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, Infectious Disease Clinic, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, Department of Biomolecular Sciences, Division of Toxicological, Hygiene and Environmental Sciences, University of Urbino Carlo Bo, Urbino, Department of Biomolecular Sciences, Division of Toxicological, Hygiene and Environmental Sciences, University of Urbino Carlo Bo, Urbino

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