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A UBI 31-38 Peptide-coumarin Conjugate: Photophysical Features, Imaging Tracking and Synergism with Amphotericin B Against Cryptococcus

[ Vol. 18 , Issue. 2 ]

Author(s):

Soraya M.Z.M.D. Ferreira, Hellem C. Carneiro, Rosemeire B. Alves, Ana Carolina S. Batista, Eufranio N. da Silva Junior, Gleiston G. Dias, Jarbas M. Resende, Daniel A. Santos, Debora L. Oliveira, Marcio L. Rodrigues and Rossimiriam P. Freitas*   Pages 157 - 163 ( 7 )

Abstract:


Cryptococcosis is a fungal disease of global significance for which new effective treatments are needed. The conjugation of the synthetic antimicrobial peptide fragment UBI 31-38 to a coumarin derivative showed to be an effective approach for the design of a novel anticryptococcal agent. In addition to antifungal activity, the conjugate exhibited intense fluorescence, which could be valuable for mechanistic investigations of this molecule. In this work, we studied the photophysical properties of the conjugate and confocal scanning laser microscopy was used to inspect the distribution of the peptide-coumarin conjugate in Cryptococcus cell. The synergism of this compound with amphotericin B or fluconazole against C. gattii and C. neoformans strains was also investigated. The results indicated that the fluorescent conjugate alone as well as its combination with amphotericin B are promising tools against cryptococcosis.

Keywords:

UBI 31-38, peptide-coumarin conjugate, fluorescent, anticryptococcal activity, interaction studies, confocal scanning laser microscopy.

Affiliation:

Departamento de Quimica, Instituto de Ciencias Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, Departamento de Microbiologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Departamento de Quimica, Instituto de Ciencias Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, Departamento de Quimica, Instituto de Ciencias Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, Departamento de Quimica, Instituto de Ciencias Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, Departamento de Quimica, Instituto de Ciencias Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, Departamento de Quimica, Instituto de Ciencias Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, Departamento de Microbiologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, Instituto Carlos Chagas (ICC), Fundacao Oswaldo Cruz (Fiocruz), Curitiba, Instituto Carlos Chagas (ICC), Fundacao Oswaldo Cruz (Fiocruz), Curitiba, Departamento de Quimica, Instituto de Ciencias Exatas, Universidade Federal de Minas Gerais, Belo Horizonte

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