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Structural Determinants in the Binding of BB2 Receptor Ligands: In Silico, X-Ray and NMR Studies in PD176252 Analogues

[ Vol. 17 , Issue. 14 ]

Author(s):

Antonio Carrieri*, Enza Lacivita, Benny Danilo Belviso, Rocco Caliandro, Piero Mastrorilli, Vito Gallo, Mauro Niso and Marcello Leopoldo   Pages 1599 - 1610 ( 12 )

Abstract:


Background: The mammalian bombesin receptor family comprises three G proteincoupled receptors: the neuromedin B receptor, the gastrin-releasing peptide receptor (BB2), and the bombesin receptor subtype 3. BB2 receptor plays a role in gastrointestinal functions; however, at present the role of this subtype in physiological and pathological conditions is unknown due to the lack of specific binders for all subclasses of bombesin receptors.

Results: Here, we present a study focused on the properties of the peptoid bombesin antagonist called PD176252, and other structural analogues with the aim to elucidate causes of their different affinity towards the BB2 receptor.

Conclusion: By means of computational techniques, based on QSAR, docking and homology building, supported by experimental data (X-ray diffraction and NMR spectroscopy) fresh insights on binding modes of this class of biological targets were achieved.

Keywords:

PD176252, Bombesin receptor, QSAR, Docking, X-ray, NOESY.

Affiliation:

Dipartimento di Farmacia- Scienze del Farmaco, Universita degli Studi di Bari , Dipartimento di Farmacia-Scienze del Farmaco, Universita degli Studi di Bari , Centre for Biomolecular Science, University of Nottingham, Nottingham, Istituto di Cristallografia, Consiglio Nazionale delle Ricerche, Via Amendola 122/o, 70126, Bari, Dipartimento di Ingegneria Civile, Ambientale, del Territorio, Edile e di Chimica (DICATECh), Politecnico di Bari, Via Orabona 4, 70125, Bari, Dipartimento di Ingegneria Civile, Ambientale, del Territorio, Edile e di Chimica (DICATECh), Politecnico di Bari, Via Orabona 4, 70125, Bari, Dipartimento di Farmacia-Scienze del Farmaco, Universita degli Studi di Bari , Dipartimento di Farmacia-Scienze del Farmaco, Universita degli Studi di Bari

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